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Core Vision

The last decade has brought about unprecedented advances in optical imaging.   The BioFrontiers Advanced Imaging Resource was created to provide a foundation for advanced imaging, enabling scientists from distinct scientific backgrounds to use emerging techniques, as well as collaborate and expand upon existing methods through research and development.

The Boulder scientific community is ideally situated to make substantial contributions to optical imaging.  Increasingly, microscopy is advancing into new spatial and temporal dimensions, borrowing existing techniques from unrelated fields in order to overcome the limitations of modern technology.  For example, the National Institutes of Standards and Technologies (NIST) and its affiliate, the Joint Institute of Laboratory Astrophysics (JILA), are now applying their knowledge on ultra-precise and ultra-stable measurements systems to biological questions.

Additionally, the ability to interact with local biotechnology companies (e.g., Amgen, Array BioPharma, Hospira, Gilead, SomaLogic, and more) provides an opportunity to share resources, and translate academic research into marketable products.

Microscopy has advanced at a tremendous pace in the last decade.  What advances in microscopy are most likely to facilitate your research?  Please provide suggestions.

Potential avenues of microscopy research include, but are not limited to the following:

  • Structured Illumination Super-Resolution Microscope
  • Multiphoton Inverted Microscope (for cellular applications)
  • Multiphoton Upright Microscope (for in vivo applications)
  • A 'BioStation' - an automated widefield imaging and cell incubation system.
  • 'Correlative Microscopy' - Combined Fluorescence and EM Imaging
  • Fluorescence Lifetime Imaging Microscope
  • Fluorescence Correlation Spectroscopy (FCS) Microscope
  • Laser Microdissection and Ablation
  • Optical Tweezers for Force Measurement
  • 'Dual-Cam' multi-EMCCD FRET measurement for full-frame 512x512 FRET
  • Second and Third Harmonic Imaging Microscope
  • Light Sheet Microscope
  • Stereomicroscope
  • Mesoscale techniques, e.g., Optoacoustic Imaging
  • Macroscope techniques - Whole animal bioluminescence
  • Stimulated Raman Microscope