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Major Equipment in the

BioFrontiers Advanced Light Microscopy Core

Director: Joe Dragavon, PhD

 

The BioFrontiers Advanced Light Microscopy Core is equipped with the following microscopes:

    Nikon A1R Laser Scanning Confocal and TIRF Inverted Microscope

      • Lasers: 405, 458, 476, 488, 514, 561, 638
      • Detectors: 4 independent PMTs
      • Widefield Light Source: Sola Light Engine
      • Motorized XYZ Stage
      • Piezo Z insert
      • Nikon Perfect Focus System
      • Environmental Chamber with O2, CO2, Temperature, and Humidity control
      • Fully motorized TIRF arm
      • Camera: Andor Ixon3 897

       

        Nikon N-STORM and TIRF Inverted Microscope

          • Lasers: 405, 488, 561, 647
          • Cameras: 2x Andor Ixon Ultra 897 and 1x Hamamatsu ORCA-Flash4.0 sCMOS
          • Widefield Light Source: Excelitas X-Cite 120LED
          • Motorized XYZ Stage
          • Piezo Z insert
          • Nikon Perfect Focus System
          • Environmental Chamber with O2, CO2, Temperature, and Humidity control
          • Fully motorized TIRF arm

           

            Molecular Devices ImageXpress MicroXL plate scanner

              • Capable of imaging 6, 12, 24, 96, and 384 well plates
              • Filter Cubes:
              Dapi YFP GFP/RFP FRET Cy3
              CFP RFP TRITC Cy5.5
              GFP CFP/YFP FRET mCherry
              • Camera: 1.4 mexapixel cooled CCD
              • Widefield Light Source: Sola Light Enging
              • Motorized XYZ Stage
              • Piezo Z insert
              • Near-Infrared autofocus system
              • Environmental Chamber with CO2, Temperature, and Humidyt control

               

                Nikon Spinning Disc Confocal

                  • Lasers: 405, 445, 488, 514, 561, 594, 647
                  • Camera: 2x Andor Ultra 888 EMCCD
                  • Spinning Disc Head: Yokogawa CSUX-A1
                  • Widefield Light Source: HBO arc lamp
                  • Motorized XYZ Stage
                  • Piezo Z insert
                  • Nikon Perfect Focus System
                  • Environmental Chamber with O2, CO2, Temperature, and Humidity control

                   

                    Olympus IX-81 Inverted Widefield Microscope

                      • Light Source: Prior Lumen 200
                      • Filter Cubes: Dapi, FITC, TRITC
                      • Camera: Hamamatsu ORCA-ER C4742-80
                      • Motorized XYZ stage
                      • Piezo Z instert
                      • Environmental Chamber with CO2, Temperature, and Humidity control

                         

                          Analysis Workstation

                            • Hardware
                              • GeForce GTX 690 graphics card for CUDA enabled GPU parallel Processing
                              • Intel 520 Series Solid State Drives for Fast Read/Write operations
                              • Seagate 7200 RPM hard-drives for storage
                              • Intel Quad Core i7
                              • 24 GB of Kinston 2400 MHz DDR3 RAM
                              • Asus z87 Delux Motherboard
                            • Software:
                              • Matlab version R2013b
                              • Imaris 8.0
                              • Nikon Elements
                              • ImageJ/Fiji
                              • ICY
                              • Olympus CellSens
                              • Cell Profiler