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User Resources

Below you will find several resources that will help you obtain the best possible results from your microscopy research.  These include tutorials that you can follow for setting up an experiment, as well as online resources that explain several aspects of microscopy.  Ultimately, we would like for this to become an online 'wiki-like' community where people from different labs and different backgrounds can effectively communicate protocols, tricks, and tips.


 

BioFrontiers Advanced Imaging Updates

Any news, or modifications to protocols, will be placed here as well as updated below.

  • 04/2014 - We have a Nikon N-STORM microscope in the facility now
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    General

  • General expectations for microscope use. - 7/1/2012
  • Microscope Scheduling7/1/2012
  • Data Transfer from Microscopes7/1/2012
  • Reporting Publications and Authorship - 8/4/2013


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    Nikon A1R Confocal and TIRF

  • Microscope Description7/1/2012
  • Directions for Turning on Microscope7/1/2012
  • Directions for Shutting Down Microscope7/1/2012
  • Directions for ND Acquisition Panel - Common to all modes of imaging (Confocal/Widefield/TIRF/Stimulation)7/1/2012
  • Directions for Setting Up Confocal7/1/2012
  • Directions for Setting Up Widefield7/1/2012
  • Directions for Setting Up TIRF7/1/2012
  • Directions for Setting Up Confocal with Stimulation (Photoactivation, FRAP)7/1/2012
  • Directions for Kohlering Differential Interference Contrast Channel7/1/2012
  • Directions for Environmental Chamber Use7/1/2012
  • Liquid Chillers for EMCCDs - 11/1/13

  • Nikon N-STORM

    • Microscope Description

     


    Zeiss Axiovert 200M

  • Microscope Description7/1/2012
  • Turning On and Off Zeiss Microscope - 8/4/2013
  • Software Directions for Zeiss Microscope - 8/4/2013
  • Filter Positions7/1/2012
  • Filter Sets for Common Fluorochromes7/1/2012
  • Zeiss Pixel Size - 7/26/13
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    Nikon TE-2000-E

  • Microscope Description
  • Directions for Setting Up
  • Filter Positions
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    Olympus IX-81

  • Microscope Description
  • Directions for Setting Up
  • Filter Positions
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    Molecular Devices ImageXpress Micro XL High-Content Screener

  • Microscope Description
  • Directions for Setting Up
  • Filter Positions

  • Sample and Reagent Preparation


       

      Online Resources